Sowmya Theegalapalli

4 Questions 4 Answers 0 Followers

Questions related from Sowmya Theegalapalli

What are some tips and tricks I can employ to revive my iPSC culture?

Hello! I'm a beginner to hiPSC culture, and while my cultures look acceptably good, I'd be grateful for any advice on how I can revive my slightly stressed culture to an un-stressed, healthy state...

04 March 2025 7,508 1 View

Why do my iPSCs keep congregating in the center of my well despite a good suspension at the time of plating?

Hello, my iPSCs always seem to migrate to the center of my well despite shaking my 6-well plate (in an infinity sign shape) quite meticulously before I stick them in the incubator. I always check...

27 January 2025 6,475 3 View

How long should I use my Matrigel-DMEM solution?

Hello. What would be the recommended duration for the usage of a Matrigel + DMEM-F12 dilution? One or two weeks? I use a "thin" matrigel coating for iPSCs - wherein I dilute about 500 ul of a...

27 January 2025 7,814 2 View

How can I tell if my matrigel coating "worked"?

Hello! I've just started to work with iPSC cell lines, and I was wondering if there are any checks I could do to ensure that the matrigel coating of my 6 well plates has gone according to plan and...

15 January 2025 6,965 2 View