I want to quantify a protein in rat uterine endometrium. As this protein may present in the other parts of uterus, this section should be separated from uterus, but I don't know how. Can any one help me?
Dissect out the uterus from the rat body, cut open the uterine tube and then gently scrape of the endometrial tissue from the uterus using a blade and collect into lysis buffer, homogenize and u r ready with ur sample.