How can I statistically analyze my -dCt values? Is it correct analyzing with 2-dct values, if we have -dCt?

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Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Subjects number in SEM for experimental design?

For an experimental design ( one experiment and one control group), at least or minimum, how many subjects need to be included in the groups in SEM?

08 January 2024 8,210 3 View

Article Removal Request?

07 November 2023 4,567 2 View

When we apply blocking solution before applying primary antibodies in immunofluorescence, how do we know it does not block the specific binding site?

In immunofluorescence technique, one of the steps in the procedure is the blocking step. We add blocking solution (universal protein block) before we introduce our antibodies, and incubate 30...

01 October 2023 4,957 2 View

How can i turn any survey result into mathematical modelling ?

What I want to ask is this: I have a survey and its analysis. I want to build a mathematical model using these analyses. how can I do it

04 September 2023 9,331 3 View

Why do we use sodium cyanide in this protocol of sod assay? What happen if i don't use?

Superoxide Dismutase Assay Method Activity of SOD has been determined in two ways: 1. Inhibition by the enzyme of an O2- dependent reaction. 2. Pulse radiolytic methods (Rigo et al. 1975) See:...

14 August 2023 3,191 1 View

If X predicts Y, and Y predicts Z; does that reflect an indirect relationship?

Hello dear researchers In my study, I have 5 independent variables and 1 dependent variable. In multiple regression analysis, not all my IVs significantly predicted my DV. However, most of my IVs...

27 October 2022 8,623 4 View

Has anyone used siRNA from Synbio Technologies before?

Hello, I am planning to do gene knockdown using siRNA. Has anyone used siRNA from Synbio Technologies before? If so, what are your thoughts?

19 March 2022 4,655 0 View

How can I transform effect sizes (cohen's d) before running a meta-analysis in R?

I'm using R to run a meta-analysis (using Robust Variance Estimation) of Cohen's d effect sizes. What transformations do I need to do and how do I do them in R? Does anyone have access to online...

13 September 2021 7,239 3 View

Why Native PAGE gel is empty?

Can you help me. I am a student Biology Department. I upload and ran the Native PAGE gel. After 3 hours, even the markers were not visible on the gel. I turned it off and did the painting, but the...

24 June 2021 2,668 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View