After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How to identify RAMAN spectrum especially for plant ?

How can I obtained plant RAMAN spectrum database for free ? or is there any useful reference can be used for the analysis of plant RAMAN spectrum ?

20 October 2019 9,268 1 View

How the type of nanoclay affecting composite's mechanical properties?

Please forgive me for without any introduction, I will try to make it short. I am understand that nanoclays can be classified as T:O (tetrahedral: octahedral ) or T:OT layer stacking . I also...

17 June 2019 7,881 3 View

Is Covaris machine ME220 suitable for yeast chromatin shearing?

I've been looking for machines that shear chromatin for my ChIP seq experiment using yeast lysate and I wonder if anyone had tried to use Covaris machine to perform yeast chromatin shearing? p.s....

31 January 2019 5,057 2 View

Can anyone suggest the electrolyte I use?

I prepared Ag-deposited material on V2O5 graphene and tried to use KOH as electrolyte. However, the resulting CV pattern does not seem to have good symmetry. Can someone suggest which electrolyte...

08 January 2019 8,404 3 View

Non-linear effects of varied predator densities?

In my current work, I found a non-linear effect of invasive predators to a native mammal community. That is, the invasive predator had a slightly positive effect to species richness of native...

05 August 2018 2,373 6 View

How many dose can we use in radiotherapy?

Hi We designed a radiotherapy study, in this experiment we used hypofraction therapy that 4 Gy per day treated local tumor and gave 4 days, the total dose is 16 Gy. However, we observed 3 of 6...

11 May 2018 6,966 3 View

Im using graphene aerogel, carbon black, PTFE(8.1.1wt%) but the product isnt chocolate sauce-like slurry. what is my problem?

Ive seen many people done it, and I use ethanol(95%) to suspend PTFE.

16 January 2018 5,461 7 View

How to generate cub file with ETS-NOCV analysis in ADF program (in PC) ?

Hi guys, I have try to repeat a published method which was called ETS-NOCV analysis in ADF program (I run in PC not in HPC). When I try to generate Cub file for Avogadro to demonstrate density...

28 November 2017 3,369 2 View

No title

21 August 2017 4,063 8 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View