How can I sequence A+T rich control mtDNA region?

More Kameshpandian Paramasivam's questions See All

While performing MD simulation, is it possible to add other ions while solvating in addition to 0.15 M of NaCl and MgCl2?

Can an MD simulation be performed by adding other salts by varying their concentration inside the box?

01 May 2023 9,230 3 View

How can be the protein immobilized s-CM5 sensor chip stored?

I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?

10 April 2023 4,319 2 View

In a MD simulation, a protein complex with > 600 amino acid can have a RMSD over 1 nm (10 Angstorm)?

MD simulation of a protein complex with more than 600 AA residues shows an average rmsd between 1-1.5 nm. can the protein complex can be considered stable? Besides sometimes i see a jump in the...

06 November 2022 6,302 4 View

Is it possible to indulge a dimeric protein (Target) into docking against another protein (Bound to a metal ion) using HADDOCK server?

I have been using CLUSPro 2.0 for protein-protein docking, but it does not take the metal ion into consideration because it selectively takes the protein chain. In contrast, HADDOCK considers the...

06 June 2022 3,285 4 View

Is there any way/tools to interepret the molecular dynamics simulation results beside XmGrace?

I have done MD simulation and want to interpret and plot the data from the files. As my PC did not have enough specs for Dual boot OS because the XmGrace tool is supported only on Linux. Though, I...

25 April 2022 4,254 3 View

Whether ethical committe (IAEC) approval is needed for samples procured from butcher shop?

I am devising a study to evaluate the invitro anti- resorptive effect of my sample in bovine bone procured from butcher shops/markets. Though there are studies in which the samples from...

20 September 2021 4,259 6 View

How to find the amino acids which are located in the particular protein binding site?

As i am working on protein-ligand docking, i want to input the x,y,z coordinates in autodock and to do that, i need the coordinates of aminoacids present in the binding site. So, PLease help me out.

03 January 2021 4,099 4 View

Importance of distance education?

Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.

25 December 2020 8,704 10 View

Farming is an non profitable occupation ? If agree / disagee reasons?

Some of the problems faced by farmers..... 1. Poor marketing channel 2. Farmers not getting remunerate price for the own produce 3. Lack of bargaining power (someone fix the price) 4. Monsoon...

30 November 2020 3,819 4 View

Difference on natural farming and organic farming???

Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...

23 November 2020 7,080 22 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Felicien Amakpe

OK dear Kameshpandian, I don't know at what temperature you are conducting your sequencing.

this may be do to the temperature conditions. please try the different temperature conditions (+-1, +-2, etc). even out of the traditional recommended ranges and it will work. hoping you the best luck

Sabine Strehl

Sequence the PCR-product/clone from both sides.