Kameshpandian Paramasivam

6 Questions 9 Answers 0 Followers

Questions related from Kameshpandian Paramasivam

What is the exact sequence for Lac Z alpha gene including promoter/operator/terminator?

Dear Sir/Mam, I am constructing vector/plasmid with Lac Z alpha gene. I have doubt the following sequence is correct to insert Lac Zalpha-...

31 March 2018 1,226 1 View

Why helper phage needed to produce microphage?

The helper phage vector was perfectly constructed for recombination, so why we have to use secondary plasmid like pLS7 or pNJB7 (Sattar et al., 2015- Ff-nano, Short Functionalized Nanorods Derived...

26 December 2017 1,805 3 View

How can I sequence A+T rich control mtDNA region?

The mtDNA-control region targeted PCR product size is ~900 bp, it is A+T (Adenine & Thymine) rich region compared to other normal mtDNA regions/genes. While sequencing (sanger) this A+T rich...

12 December 2015 367 2 View

How can I extract DNA from 50 to 100 years old museum preserved/dried bird samples?

I used DNeasy (Qiagen) and invitrogen DNA mini extraction kits with digestion modification, but I am not getting DNA's. So please anyone can give me the protocol or kit to extract DNA from 50 to...

02 April 2015 1,039 7 View

How can I convert mega or fasta file format into *.rdf file format for constructing median joining network in Network software?

I have aligned nucleotide sequences in mega format, I need to construct Median Joining network in Network software. Please anyone can tell me how to make *.rdf file and how to construct MJ network...

23 March 2015 7,215 9 View

How do you make an Arlequin input file for AMOVA?

I have aligned cyb sequence of four population. I don't know how to convert raw(fasta) or mega file to Arlequin (.arp). and I dont know how to create profile name/sample group anything following...

16 March 2015 5,789 10 View