I want to create Amphiphilic Polysaccharide and PLA MPs by electrospray technique (single axial). Could you suggest suitable solvent ?

I know that PLA is soluble in DCM, not soluble in water and hard to dissolve in DMF. What kind of solvents will be suitable for electrospray system for the amphiphilic polysaccharide and...

13 October 2020 3,559 4 View

Does anybody look Erasmus K2 Projects Partnership?

Hello All, We are currently looking for Erasmus K2 Project partner in Europeen countries. Are there anyone interested in becoming a project partner in the social sciences and (or technical...

10 December 2019 6,491 2 View

Ethanol contamination during elution of cDNA with TE in RNA-seq library prep?

I am using NEBNext Ultra II RNA library prep kit for preparing libraries from bacterial samples. When the libraries ready, I did second Ampure bead purification for those have dimer peaks. During...

24 October 2018 8,971 2 View

Multi step ahead time series forecasting in SVR?

Hi, I am trying to do multi step ahead time series forecasting by using SVR. When I do forecast for training dataset, the results are pretty good. However, when I do iterative forecasting by...

24 July 2018 4,713 3 View

Which way is better for batch anaerobic reactor? Continuous nitrogen sparging or one time sparging?

I am trying to grow Bifidobacterium longum in a anaerobic reactor. I use MRS+ L-cysteine for the growth media (which is also regular growth media). I maintain anaerobic conditions with N2...

07 March 2018 9,449 7 View

Protein isolation and western blot from FFPE tissues?

we tried to isolate and western blot proteins from Formalin fixed paraffin embeded tissues. We did this protocol: 1. we got 5 x 10 micro meter paraffin sections. 2. xylene, 96-90-80-70 alchols,...

23 August 2017 9,064 3 View

I am using NEB 10-beta competent E.coli cells. Transformation is successful but colonies dont grow in LB. Do you have any advise?

I am using NEB PCR cloning Kit. There is pMiniT vector inside kit. I transformed ligation bwt my PCR product and pMiniT vector to NEB 10-beta competent E.coli cells. I used SOC growth medium for...

21 February 2016 2,608 7 View

Does anyone have any experience in ITK-SNAP for muscle DTI analysis and fiber tracking?

04 November 2014 1,459 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View