How can I validate the UV spectroscopy?

can I compare UV spectroscopy reading with nanodrop reading to validate the UV spectroscopy? or is there a better way to know if the UV works well or not? Note: we use it in DNA, RNA &...

12 December 2020 4,869 2 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

What's makes RNA (and not DNA) bind to the silica spin column in commercial kits?

In silica spin column-based kits, if the spin column material is the same and the binding to the column occurs by using ethanol and Guanidine salts. Then, what's the difference between the RNA...

20 September 2020 4,798 1 View

Is it possible to apply statistics on synthesis and anticancer activity?

I m looking for paper having knowledge of statistics (spss or matlab) applied on synthesis and anticancer activity

20 March 2020 5,517 3 View

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

09 December 2019 7,276 3 View

I would like to know about how can crystallization of metal complexes take place?

When i prepare the complexes it forms in amorphous state i want to make crystals of complex.my complexes soluble DMSO but when i dissolved in it and pour insoluble solvent it was oxidized.

15 February 2019 7,053 2 View

Help Please!! how to solve the error "too many attempts made for this increment" in abaqus?

i am applying a load of 50KN gradually on a reinforced concrete beam, i'm unloading this load using an amplitude to get the loading and unloading versus deflection graph.i have used concrete...

09 June 2017 3,129 14 View

Hello How to get a producer to dissolve cellulose fiber by carbon disulfide ?

11 April 2017 9,899 2 View

How to get the frequencies of simply supported RC beam when changing load in ABAQUS?

i have modeled the RC beam with simply supported BC and used concrete damaged plasticity model to account for the nonlinearities in concrete, i have got the frequency value for the case of beam...

01 January 2017 737 6 View

What kind of experiment help in understanding the metabolic pathway in plants?

In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...

18 September 2016 396 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View