I isolated genomic DNA of Musca with one commercial kit. The 260/280 ratio is 2, so I supposed there is a RNA contamination. Please, I would like to know if there are methods to remove RNAs without using RNAse. İf yes, wich methods?.
If you really think RNA to be interfering with your reactions, you can re-purify your extracts with alkaline-Phenol-chloroform method. Alternatively you simply run your extracts on an 0.8% agarose gel, slice the gDNA band from the gel and purify with a gel DNA extraction kit.