How can I preserve fungal culture for a long time?

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Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

Query Regarding the Impedance EIS Data?

Respected All Research Members. Actulaay I faced the problem while analysis the EIS Data as i got the value of Z" negative. What should be the possible reason for this. Thank you in advance

03 July 2024 4,336 3 View

Can anyone suggest an adhesive that can sustain 600C?

I have to join tungsten wire to metallic plate with that adhesive and entire assembly has to be heated upto 600C in ultra high vacuum. I know about zirconia paste but it is not readily available...

18 June 2024 3,092 3 View

Kindly help me to identify this fungal slide ?

fungal inoculum treated with lacto phenol blue and observed under microscope anyone help me to identify and share some information regarding this fungal slide

07 June 2024 9,647 3 View

How the dropped packet is being transmitted in RPL DODAG?

I am implementing an enhanced objective function for RPL. Control messages and data packets of UDP applications are being transmitted. Now the question is that if any data packet which is being...

02 June 2024 3,418 2 View

In what specific applications are rotating heat pipes most advantageous compared to traditional heat pipes?

Explain your ideas

07 May 2024 4,553 2 View

Will it cause any problem to take 7% Margin of Error for determine sample size in Stratified Sampling Technique?

Greetings, I am Mamun. I just want to know that if I use 7% margin of error than will it cause any problem in future analysis? I am using stratified sampling technique, for this if i take 7%...

06 April 2024 2,344 6 View

What are the openly available data sources that may be gathered and used for research publications?

02 March 2024 6,825 3 View

How do I remove a publication that is not mine from my account?

In opening up my account, I accidentally picked publications that are not mine and I would like to remove it from my account. Please advise/remove them. and also close this account. I am running...

27 February 2024 7,549 0 View

Is it possible to get three or more than three kids/offspring from a single goat/doe as she comes in heat and met by more than two bucks, at a time?

simultaneously

24 February 2024 284 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View