I have two bottles of cell culture media MEM Hanks and 199 Earls
I dont need them now, but i will later. How can I preserve them for about 6 months , without loss in nutrients or contamination ?
Hello Reham.
That is not a good idea to store media containing many different chemicals in them. However, if you must follow the procedure below.
Start from newly arrived fluidal or made from the powder formulation. Take great care of absolute sterile condition to make aliquots (50 or 100ml each in plastic container without serum without glutamate or pyruvate. Freeze them and store at -70C. When you need to use them, thaw in a water bath. Bring them to lamina flow. Supplement a half of Glutamate or puruvate if the media were used and stored at 4C for some time already. Otherwise, no need to supplement the energy sources. Millipore and add serum, followed by milipore again. They are ready for use.
Best wishes.
Hello Reham.
That is not a good idea to store media containing many different chemicals in them. However, if you must follow the procedure below.
Start from newly arrived fluidal or made from the powder formulation. Take great care of absolute sterile condition to make aliquots (50 or 100ml each in plastic container without serum without glutamate or pyruvate. Freeze them and store at -70C. When you need to use them, thaw in a water bath. Bring them to lamina flow. Supplement a half of Glutamate or puruvate if the media were used and stored at 4C for some time already. Otherwise, no need to supplement the energy sources. Millipore and add serum, followed by milipore again. They are ready for use.
Best wishes.
ya its not a good idea to use your old culture media.
some properties may change. u will not get a better result.
Hello Reham,
I presume from your question that you have two bottles of reconstituted media and that you have already added serum and glutamate supplements. If that is the case, there is nothing much that can be done. Deep-freeze it and after 6 months, before using, add more glutamate (~50%) and check sterility.Then, proceed as you would otherwise. If that is not the case, let me know so that I may provide a better answer. In general, you may recall that glutamate undergoes gradual, temperature-dependent breakdown and produces toxic residues. Hence, the performance of your media after 6 months will be better if you have not added glutamate already or if you have used glutamate replacers.
Best wishes.
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