I will add these drugs in 20 mL organ bath. The maximum volume in the bath is 20.11 mL. I wonder about osmolarity of solution in the bath.

  • If I prepare these drugs in distilled water and ascorbic acid (to protect oxidation), the osmolarity in the bath will decrease from osmolarity of Krebs-Henseleit solution right.
  • On other hand, If I dissolve the drugs in PBS and ascorbic acid, the osmolarity in the bath will equal the osmolarity of the Krebs-Henseleit solution right.
  • What is the choice that I would choose?

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