How can I prepare a synthetic substrate solution for amylase activity?

More Neeta sudamrao Khillare's questions See All

Can someone please help me with Gpower for sample size ?

David Morse you helped me previously, please can I check with you my question is : the impact of working from home on loneliness, creativity and resilience. I know I will be using multivariate...

28 September 2021 3,434 3 View

I need some help with Multivariate Regression and sample size using G Power?

I'm very new to this and have 1 Independent Variable ( Working from home) and its effect on 3 dependent variables ( loneliness, creativity and resilience) . Can someone please confirm I will use...

26 September 2021 598 3 View

How does student funding for higher education help in human capital formation?

Views and comments please...

06 May 2019 1,871 0 View

How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

09 September 2016 2,633 6 View

How can i simulate different data encryption algorithm using CloudSim?

CloudSim is a simulation tool for cloud computing but i m not getting that where i insert my encryption coding for simulation. Please help me regarding this if possible provide any example also

19 June 2015 8,974 8 View

Can you advise me on the high efficiency transfection of the cells in the suspension?

I am working with the transformed bovine lymphocytes. These cells are in the suspension and I am planning to transfect these cells with the gene of my interest. Cell in suspension is often hard to...

12 February 2015 6,105 1 View

How can I prove quantitatively that all my cells are expressing GFP?

I have gfp expressing cells. Almost all of the cells are expressing GFP. But somehow the intensity of flourescence is very low and therefore it cannot be detected by FACS. Can someone tell me...

06 November 2014 4,784 5 View

What is the time point for the observation of global impact of viral miRNAs on transduced cells?

My experiment is related with miRNAs. I would like to see the effect of miRNA on transduced cells. So the idea is that miRNA after causing obstruction of protein production should show subsequent...

03 September 2014 1,575 3 View

Are there any specific guidelines for managing thrombocytopenia due to everolimus in a breast cancer patient?

A patient of metastatic breast cancer on exemestane and everolimus gets prolonged thrombocytopenia (30,000) that responds only partially to platelet infusion, even after everolimus has been...

19 June 2014 138 1 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

27 September 2013 9,925 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View