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Questions related from Neeta sudamrao Khillare
I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...
10 September 2016 2,665 6 View
I have very less (in mg) quantity of synthetic substrate(p-nitrophenyl alpha d-maltopentaoside).help me to set up a protocol for amylase activity assay. I am working with insect amylase.
12 June 2015 6,439 3 View
There are some papers for artificial diet but there was no growth observed during my work. The insects were dead after 2-3 days survival. Maintenance of relative humidity (27-30 degree Celsius)...
13 January 2014 1,559 0 View
During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.
28 September 2013 9,947 4 View
I have to grow it in an artificial medium.
28 September 2013 2,440 8 View
I have extracted the enzyme from a biological source by using distilled water.
09 August 2013 5,584 3 View
I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.
27 June 2013 5,405 8 View