Neeta sudamrao Khillare

6 Questions 7 Answers 0 Followers

Questions related from Neeta sudamrao Khillare

How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

10 September 2016 2,597 6 View

How can I prepare a synthetic substrate solution for amylase activity?

I have very less (in mg) quantity of synthetic substrate(p-nitrophenyl alpha d-maltopentaoside).help me to set up a protocol for amylase activity assay. I am working with insect amylase.

12 June 2015 6,393 3 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

28 September 2013 9,906 4 View

Can anyone suggest to me a method for identification of male and female Tribolium castaneum?

I have to grow it in an artificial medium.

28 September 2013 2,408 8 View

How to calculate enzyme activity of extracted amylase?Is there any specific method other than DNSA method?

I have extracted the enzyme from a biological source by using distilled water.

09 August 2013 5,543 3 View

If we will increase the percentage of ammonium persulphate for polymerization of NATIVE PAGE, is there any effect on % of gel?

I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.

27 June 2013 5,373 8 View