Neeta sudamrao Khillare

7 Questions 7 Answers 0 Followers

Questions related from Neeta sudamrao Khillare

How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

10 September 2016 2,669 6 View

How can I prepare a synthetic substrate solution for amylase activity?

I have very less (in mg) quantity of synthetic substrate(p-nitrophenyl alpha d-maltopentaoside).help me to set up a protocol for amylase activity assay. I am working with insect amylase.

12 June 2015 6,444 3 View

Is there any easy method for rearing (without incubator) flour beetles and artificial diet in laboratory?

There are some papers for artificial diet but there was no growth observed during my work. The insects were dead after 2-3 days survival. Maintenance of relative humidity (27-30 degree Celsius)...

13 January 2014 1,562 0 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

28 September 2013 9,954 4 View

Can anyone suggest to me a method for identification of male and female Tribolium castaneum?

I have to grow it in an artificial medium.

28 September 2013 2,443 8 View

How to calculate enzyme activity of extracted amylase?Is there any specific method other than DNSA method?

I have extracted the enzyme from a biological source by using distilled water.

09 August 2013 5,587 3 View

If we will increase the percentage of ammonium persulphate for polymerization of NATIVE PAGE, is there any effect on % of gel?

I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.

27 June 2013 5,407 8 View