How can I prepare a potassium phosphate buffer 0.05M and pH 8?

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Kong M Li

You can either: (1) prepare 0.05M KH2PO4 and 0.05M K2HPO4, then mix/titrate to get the final pH of 8. (2) Use Henderson Hasselbalch equation to calculate the mole fractions of KH2PO4 (Y) and K2HPO4 (X), You have two unknowns X and Y but you also have two equations 0.8=log (X/Y) and X+Y = 0.05M. Hope it helps.

Mrinmoy Sarker

Dissolve 0.68g of potassium phosphate in 9.0ml of DDW. Adjust pH to 8.0. Then make up the volume upto 10.0ml.

Use S=(1000W)/MV

S=concentration.

W= weight of compound

M = molar mass

V=volume required.

this equation for molarity related calculations.