Please explain me is it possible from cacodylic acid other than sodium cacodylate
Here is a receipt from "buffers". Basically, make 0.2 M cacodylic acid and 0.2 M NaOH. Add 0.2 M NaOH to 0.2 M cacodylic acid to desired pH.
0.2M cacodylic acid 1 liter
(CH3)2AsO2H (MW = 138.0) 27.6 gm
+ ddH20 to make 1 liter
0.2M NaOH 100 ml
NaOH (MW = 40) 0.8 gm
+ ddH20 to make 100 ml
Working buffer: 0.1M
Adjust 50 ml of 0.2M cacodylic acid to desired pH with 0.2M NaOH. Dilute to 100 ml with ddH2 or dilute 1:1 with fixative.
http://www.ou.edu/research/electron/bmz5364/buffers.html
Starting from cacodylic acid you could add sodium hydroxide to make a cacodylic buffer. This would get you to the same point as adding cacodylic acid and sodium cacodylate. You either use half the molar amount of sodium hydroxide to get right in the middle of the buffering range. Alternatively just titrate it to the right pH.
Dear Dhanasekar,
as Ying I would like to provide "data" out of my collection on how to make "cacodylate buffer" not knowing which purpose it should serve.
In the attached pdf's you'll find perhaps also "your" solution....
best wishes and good luck, Wolfgang
Dear Muss,
Thank you for your suggestion, i need to prepare the cacodylate buffer for preparing 2.5% Glutaraldehyde in cacodylate buffer, and also use to wash the sperm sample for SEM study after fixation.
Dear Dhanasekar (assuming Krishnamoorthy your family name....apologize for my informal entitling you with your first name as this is usual in RG...but done honestly and respectfully!)) ,
You're welcome.
If you need proper recipe or any help for this, just tell me the ingredients you have in place. Remember or have in mind that probably need some "additives" in the fixative (like Ca++, or even Mg++) and the fixative perhaps needs also adjustment to tonicity (+ saccharose)....There are some very specific recipes for sperm-spec-prep out in the wild....(:-))
Bst wishes and regards,
Wolfgang
(now out of the Lab)
Dear Wolfgang,
sorry to call like muss,
Thank you very much for your kind response.
Krishnamoorthy is my father name.
i've cacodylic acid, 25% glutaraldehyde and paraformaldehyde in EMS grade from Sigma. my fish is marine animal.
other than if you tell me means i'll check it.
With thanks,
Dhanasekar K.
Dear Dhanasekar, I am sorry for perhaps overlooking this your last reply for long.... Most obviously I didn't get the usual ResearchGate-notification for new reply(-ies) in time.... Since today I got such for the new recommendation of my reply No 003 (THANK YOU to Pierre-Louis!) I wondered what my recommended reply were...
As for the quest on the use of "cacodylic acid" I guess you've made it and found all the information necessary on how to make Cacodylate buffer from Cacodylic acid.
So right now I became aware of the fact that the protocols I added (at least the file BUFFERS,General considerations...) essentially is the same as provided by Ying Henderson which honestly I today like to recommend also. I am sure too that you managed meanwhile your study on fish sperms and preparing your primary fixative 2.5% GA in 0.1M Cacodylate buffer.
Good luck, best wishes and regards, W.M.