Aimée Obolari Durço

2 Questions 3 Answers 0 Followers

Questions related from Aimée Obolari Durço

How can I perform total protein normalization in a QWB using Ponceau S?

I've always used abd as load control for western blot normalization, and use Ponceau S stain as a transference control (along with keeping an image - a picture taken by my cellphone - for...

29 February 2024 1,498 2 View

How important is matching the molecular weight of my protein of interest with the ladder?

Hi everyone I am working with a 60kDa protein (according to the datasheet), in a 10% SDS gel. The membrane shows 3 sets of double bands, and the one in the middle is faded compared to the other...

27 January 2023 7,830 2 View