Dear all, I'm planning for continuous overexpression and Knockdown.
I'm using Cre-ERT2 system, and i want to firstly overexpress YAP and then knockout YAP in lung.
i have made loxp-YAP-loxp, so it is possible to knock out YAP, but i have no idea how to overexpress YAP before Tamoxifen (for Cre-loxp system) injection
Do you have any idea for overexpressing YAP, not disturbing Cre-loxp system?
I'm considering tet-Doxycycline system, but i can't design the experiments
Thx for your help.