I need the mi-RNA profiling of radio-resistant gastric cancer. How can I obtain it?
Try NCBI GEO database
I use salting out DNA extraction methods . But recently after adding the ethanol we couldn't saw the DNA skein and DNA pellet . Finally the results of nano drop indicated that extracted DNA have...
08 September 2019 2,017 1 View
Recently we decide to evaluate the miRNA expression levels in saliva sample as diagnostic biomarker of cancer . We used TRIzol extraction methods but the CT of real-time PCR for U6 gene was...
08 September 2019 1,548 3 View
Recently we evaluate the serum IL-6 level in patients after radiotherapy . Unfortunately 4 of 7 samples are negative. we used the thermo fisher ELISA kit for this aim and 100 microliter of...
07 August 2019 2,793 5 View
I want to evaluate the ionizing radiation effects on miRNA expression level in mice. Some paper used PBMCs and others used whole blood ( WBC ) for RNA extraction . is there difference between...
04 May 2019 8,140 5 View
unfortunately during this month i couldn't access to the miranda microRNA target prediction tool web page . http://www.microrna.org/ is the web page address was changed ?
05 June 2018 9,454 0 View
I want to design primer for the lncRNA entitled nonhsat062994 but i can't find it at NCBI. how can i access to the sequence of this RNA?
04 May 2018 8,787 4 View
My friend sent me the agarose gel results. As seen in attached figure lower range of gel was dark and , i cant see any band in this region . how can i solve this problem?
11 December 2017 5,031 0 View
Recently two study indicated that mineral oil can be used for Dna extraction. Is there anyone who has the experience about this method?
10 November 2017 4,348 5 View
Recently we want to target a gene with siRNA , but i have a critical question how can i make sure that downstream pathway of this genes will be affected . on the other hand ho can i predict...
10 November 2017 2,047 3 View
I run the sds page but after transfer to membran and staining I saw that lower band are biger than higher band. How can i solve it?
08 September 2017 7,942 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...
15 July 2024 762 3 View
Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...
14 July 2024 936 0 View