Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

The use of n-of-1 studies for rehabilitation / therapies (i.e. non-drug/medicine studies)?

Hi there, I am eager to hear from researchers or clinicians who have used n-of-1 studies for the above purposes. They seem to offer advantages over many other study designs, particularly for when...

26 October 2020 328 5 View

How can we homemade measure the pH from a soil sample?

How can we homemade measure the pH from a soil sample? I have two soil samples and I'm looking forward to knowing how can I measure the pH without technologies. "pH is determined by measuring the...

15 September 2020 5,466 11 View

PLS-SEM or CB-SEM after qualitative work?

Hello, My research team completed extensive interviews/triads with participants on various topics. The quantitative survey is the next step in our process. From that survey we want to prioritize...

13 August 2020 828 5 View

Are there examples of non-native insects that respond negatively, or neutrally, to anthropogenic disturbance?

I've combed the literature, but am having a hard time finding any examples. Specifically, I am looking for any examples of non-native insects that respond negatively, or neutrally, to...

30 July 2020 8,920 6 View

Nanotechnology question. Getting rid of solvent after LNP formulation?

I'm dealing with really micro volumes and I'm trying to figure out if there are other efficient methods aside from dialysis to get rid of residual solvent for LNP purification. And if not, are...

25 May 2020 6,377 1 View

Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

If two drugs activate the same apoptotic pathways, can they have an antagonistic effect?

Hello! I'm testing the photochemotherapeutic potential of a drug in vivo. It turned out that treatment with the prodrug caused an effect that was similar to the effect obtained after...

10 February 2020 3,001 3 View

How do you conduct a factor analysis with Multiple Imputation Output in SPSS?

I am working on my doctoral dissertation and have hit a road block with my factor analysis. I used multiple imputation for missing data (n=198 with 5% missing data), but now I am unable to figure...

05 February 2020 6,437 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View