What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

How to find degree of deacetylation of chitosan colorimetrically?

I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...

27 January 2021 8,532 2 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

DNA/RNA G4 modelling and visualization tool ?

Hi, I would like to ask if anyone knows any tool for visualizing/modelling guanine structures (any RNA structures with Hoogsteen base pairing) based on sequences? I mean, if there is any...

02 November 2020 1,136 2 View

Why do we use klenow fragment for sequencing?

I came across klenow fragment when I was reading about HiTS-FLIP method. I know klenow fragment doesn't posses 5'->3" exonuclease activity but what advantage does it provide for ?

01 November 2020 7,598 3 View

What is the best primer-design database and how to choose the best pair of primers i.e. what are the optimal characteristics?

Characteristics: product length, GC content, base repeats, melting temperature, exon-exon junction vs including an intron, self and 3' complementarity, the specificity of the primers, primer...

14 October 2020 841 7 View

How to cluster the ORF sequences?

Hi, I have found the ORF region of my nucleotide sequence which was obtained from Sanger sequencing using 16s universal primer. I have to cluster them before using them in my model. I am using...

13 August 2020 1,079 4 View

Can anyone tell me a good PCR reaction for a fragment that is about 200 base pairs?

I am struggling to get a high quantity of DNA after I perform PRC on my sample. I was wondering if anyone could suggest a good PRC reaction (temperatures and times) for a fragment of that size.

30 July 2020 5,264 14 View

Why are the ClustalW (codon) and MUSCLE (codon) alignment options greyed out in MEGA-X?

Hi, I'm currently trying to do a multiple sequence alignment for a gene family using the coding nucleotide sequences. I want to create an alignment based on the amino acid sequences but that is...

28 July 2020 2,709 3 View

What should we do if?

if we do not get appropriate GC content and melting temperature while designing primers for full gene amplification? i have a nucleotide sequence of sars cov2 surface glyco-protein of which i have...

20 July 2020 8,085 2 View