I want to design a novel peptide drug with 7-9 aminoacids. how can i determine the stability of it in human serum by HPLC method? how can i recognize the percentage of peptide in serum while it is novel and has not any antibody?
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
The Tianjin-Yonghe's cable Bridge in china The repair of this bridge was done between 2005 and 2007
07 January 2024 3,940 0 View
Any structures like the bridge, building,..... It is better if the structure is reinforced concrete. For damage detection using machine learning.
15 September 2023 4,129 3 View
i am looking for efficient texture analysis software for MRI images analysis with free access?
09 October 2020 3,931 2 View
Hi everyone, what is the voltage of the electrical potential of the cell membrane when a seizure starts in epileptic patients? and what is it in the normal cell? ( before seizure) I want to know...
15 April 2020 8,006 0 View
hi everyone. what is the molecular weight, log P and cargo size of a peptide that cross BBB? hoping that help me with answer these questions. thanks :)
20 February 2020 648 5 View
I am comparing different emulsions freeze-thaw stability using TA Q-1000 with aluminum pan, around 15 mg of liquid samples (emulsions). I expected to see an exothermic crystallization peak...
25 July 2019 5,877 5 View
Hello to all the members of the world of science! It's a tensile-related question and kind of a basic one; but I'd really like to know the answer. So here we go: Notwithstanding the fact that for...
09 October 2018 9,177 4 View
The two materials are very complicated polysaccharide and proteins. So, vibration is somehow expected. However, after each exothermic reaction, an endothermic one is occurring; which I do not have...
06 September 2018 6,790 6 View
Can any one advise what software package is best to simply deform part/all of a 3D model, without going through all the hassle of surface editing, etc.? Background: 1- I have a nominal 3D model...
29 May 2018 2,866 7 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View