I want to measure the amount of inorganic nitrate and phosphate retained by a single individual wetland plant in a field. Are there any reliable and cheap methods to do this?
Hi all, I have a plasmid with "LTR >> EF1alphacore promoter > protein1-T2A-tagBFP-P2A-BlasticidinResistance >> LTR". I made stable murine 3T3 cells, using Blasticidin 20µg/ml,...
29 August 2023 1,051 4 View
Hi all, I have tissue samples (lymph nodes) from mice, which constitutively express GFP in some cells (which I am not interested in). I usually fix and freeze the tissue in OCT. is there a way to...
22 February 2023 5,230 1 View
Hi all, I am planning on NP-imunisation of mice to study B cells. However, when looking at suppliers, there are various ratio's available. Which one is recommended, or what is the rationale to...
12 January 2023 2,854 2 View
Hi all, I am transducting primary mouse splenocytes, and was wondering if there is another method to determine the quality of the (in HEK293T) produced lentivirus? As when doing a titration in HEK...
12 October 2022 3,392 1 View
hi. i have a peculiar problem while culturing PBMC from human as well as mice splenocytes. for the picture part, I isolated T-cells using Ficoll-paque gradient (sigma), and then depleted...
21 July 2020 8,535 3 View
Are there today any easy free GUI software e.g. to do with DEM this: draw a polygon, or select a feature/features/, or (automatically) somehow determine a wider area, and get the length, width...
14 July 2019 5,096 7 View
I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...
30 May 2019 3,349 3 View
Hi, I am experiencing an absurd problem while doing FACS. While staining OVCAR-3 cells with CD90 marker (for titration), i found that antibody washes away after centrifugation (which I guess...
14 October 2018 1,579 4 View
I am doing a small quality analysis of a ceramic slip-casting process. I'd like to find out how many percentage of the quality faults, in general, is caused by different phases of the slip casting...
05 October 2016 5,417 3 View
Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...
05 August 2014 3,498 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?
31 July 2024 3,965 0 View
How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?
31 July 2024 368 2 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
How do bacterial species contribute to ecosystem functions and nutrient cycling and role of microbial communities for sustainability?
25 July 2024 2,351 2 View
Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...
23 July 2024 7,819 5 View
I examined telmisartan dissolved in methanol with a mobile phase of formic acid:acetonitrile (8:92) giving a retention time of 3.8. However, when I added acetonitrile to the solution the retention...
19 July 2024 5,462 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
18 July 2024 5,225 3 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View