I nnee dI need to measure levels of insulin in the blood samples of females with PCOS. What are the better methods available and what kits should I use. Suggestions are most welcome.
Hi
ELISA would be the best method. Kits are available.
(Cat#: ELH-Insulin-001).
You can use Elisa Kits. You can find more in this link:
https://labtestsonline.org/understanding/analytes/insulin/tab/sample/
What are the best kits available? What elisa kits would you recommend for better faster and consistent results
I often ask for serum insulin tests with PCOS patients and too often vials are not immediately put on ice. I have read this compromises the test and leads to negative results. How much of an issue is this and how often does lack of icing compromise studies that look at insulin levels and function?
Commercially available radioimmunoassay (RIA) kits are the best method for measuring serum insulin levels.
Research conducted so far shows that oral glucose and fbs using diagnostics testing is the most profound way.
http://press.endocrine.org/doi/abs/10.1210/jcem.83.8.5054
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