Comet assay is a good technique to check the DNA damage.

Thanks

Hi Mr. Hormaechea-Agulla,

You can also use flow cytometer with H2AX detection kits. Gamma H2AX is great biomarker for DNA double-strand breaks.

Agree with Okan. FACS is much quantitative and reliable. Plus less work than comet assay.

Remzi Okan Akar Susara Madduma Hewage Thank you for your responses. My laboratory use a lot flow cytometry some seems to be a good approach for me. Could you recommend me some reliable kits for gamma H2AX detection by flow cytometry?

Thanks!!

Hi Daniel,

Use Anti-gamma H2A.X (phospho S139) antibody (ab11174) from abcam. They have given few reference articles who used their product for WB, immunofluorescence and flow cytometry.

Article Zhang YW, Regairaz M, Seiler JA, Agama KK, Doroshow JH, Pomm...

But I must say that I haven't used this product. But it's a straight forward assay. You will get what you are looking for.

Cheers,

Susara

Hello again,

I agree with Mr. Hewage. You can Also I can suggest you another alternative:

MCH200101 | Muse® H2A.X Activation Dual Detection Kit.

We used this kit according to manufacturer's protocol with Muse Flow Cytometer.