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Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...
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I did a blot with HMG Co-A Reductase primary antibody (Cat # BS-5068R) purchased from Bioss (Woburn, Massachusetts, USA) for mouse liver tissues. I am getting a band at 70kDa, though the protein...
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Hello all, I am looking for a kit or a method to measure beta oxidation of fatty acids (without radio labeling) in tissues (Mouse) and cells (Human). Any suggestions ? Thanks
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I was trying to detect H2O2 induced intracellular Reactive Oxygen Species (iROS) generation with DCF-DA fluorescent dye (2′,7′-Dichlorofluorescin diacetate, Sigma Aldrich, St. Louis, MO) in HK-2...
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Has anyone tried out Sekisui diagnostics triglyceride assay kit to determine mouse liver triglycerides ? Though the Sekisui protocol is made for automated systems I'm trying to use it manually....
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