Susara Madduma Hewage

4 Questions 27 Answers 0 Followers

Questions related from Susara Madduma Hewage

Have you ever got high backgrounds in western blots done with heart tissues ?

Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...

12 June 2019 2,833 3 View

Measure beta-oxidation of fatty acids?

Hello all, I am looking for a kit or a method to measure beta oxidation of fatty acids (without radio labeling) in tissues (Mouse) and cells (Human). Any suggestions ? Thanks

17 January 2018 8,963 11 View

Getting low fluorescence values for Control group compared to H2O2 group in intracellular ROS detection with DCF-DA probe?

I was trying to detect H2O2 induced intracellular Reactive Oxygen Species (iROS) generation with DCF-DA fluorescent dye (2′,7′-Dichlorofluorescin diacetate, Sigma Aldrich, St. Louis, MO) in HK-2...

10 November 2017 1,657 5 View

Has anyone tried out sekisui diagnostics triglyceride assay kit ?

Has anyone tried out Sekisui diagnostics triglyceride assay kit to determine mouse liver triglycerides ? Though the Sekisui protocol is made for automated systems I'm trying to use it manually....

11 October 2017 3,590 0 View