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In our lab, some cell lines are dying and some cells were stay alive after trypsinazation. For example, A549 cells are normal after thaw them from -80. Subculture them and then we found them dead....
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Hello, Is there a database about which proteins transcribed by which transcription factors and also if my target protein is a transcription factor, it transcribes which proteins?
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I did IP (Immunoprecipitation) and all steps of western blotting. After the result i try to use Ponceau red to detect proteins but evertime when use it for IPed proteins on membrane it didn't...
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