5 Questions 20 Answers 0 Followers
Questions related from Remzi Okan Akar
In our lab, some cell lines are dying and some cells were stay alive after trypsinazation. For example, A549 cells are normal after thaw them from -80. Subculture them and then we found them dead....
07 October 2018 7,488 3 View
Hello, Is there a database about which proteins transcribed by which transcription factors and also if my target protein is a transcription factor, it transcribes which proteins?
24 August 2018 8,233 3 View
I did IP (Immunoprecipitation) and all steps of western blotting. After the result i try to use Ponceau red to detect proteins but evertime when use it for IPed proteins on membrane it didn't...
04 August 2015 1,708 8 View
We are doing IP isolation for the first time and we had found different protocols, but understand that we have a problem. Can anyone suggest what is the best protocol or provide any other...
03 December 2014 2,615 6 View
When doing transfection, do the cell membrane's cationic and anionic faces affect the endosome for my reagent? I mean if my cells' membrane's outer face is cationic and my reagent outer face is...
25 October 2014 6,488 5 View
