Hello everybody. I am doing some experiments of cell tracking using light microscopy (phase contrast) sequential images. I have used the following plugins of ImageJ for this: Manual tracking and Chemotaxis tool. They are OK to analyze velocity and distance. But now I want to consider the cell shape (volume, area..) and see how this value changes on time.
How can I select one specific cell and follow its modification in shape? Do I need to do segmentation? How can I do it? Is there any ImageJ plugging to do it?. Thank you in advance for your help. All the best. Alex
Hi Alex,
Please find attached a paper that might helps!
All the best,
Younes,
Transmitted light analysis (DIC, phase contrast) is always difficult because the contrast of the cells against the background is not sharp like it is with florescence. I have used the variance filter in ImageJ to pick out cells for measurement before. It works OK. You can see the other topic where I described the procedure here: https://www.researchgate.net/post/How_to_do_confluencey_for_flat_cells_in_ImageJ
you can do computer vision feature based template matching, and quantify the change from the template cell (which can be shape, texture, etc. changing for the cell). you would need to isolate the cell, either manually or using other 'cell detector' functions to do so automatically, which also makes the algorithm more complex.
Thanks Younes. I already have this publication. The FracLac plugging for ImageJ works very well but it is a bit hard to analyze 40 cels in a film with 91 frames. I was trying to find an easier protocoll. One again, thanks for your help.
Alex
Hello Christopher. Your protocoll is very good. I will try to process all the images (in a batch) and see what happens. Thank you for your imput.
Hello Vikas. Thank you for your solution. I will also explore your protocol.
Hi Alex, have you contemplated the possibility of using a circularity index? It is an easy way of giving SOME information about cell shape changes
Hello Monica!!! I have also consider this option. The point is to get an easy and fasy protocol to analyze a lot of cells , treatments... Thank you for your answer.
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