What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

I want to treat the HepG2 cells with either T3 or GC1 (TR-beta1 agonist) for 24 hours. I'm not sure about the internal control for qRT-PCR, in order to accurate normalisation of gene...

20 July 2019 5,877 3 View

How can I reference a translated book that I have contributed as part of a team work in order to add to my CV publication list?

I'd like to add a translated text book which its translation has been done by me and other contributers , to my publication list of my CV. I'm not sure about how could I reference that? Shall I...

01 June 2019 1,990 6 View

How to model the Voids in a rock with zero elastic properties in Abaqus ?

Hello, I am trying to build a model that represents a rock with different minerals in Abaqus to recover the elastic properties like the image. My question is how to define the voids as a...

19 May 2019 6,362 7 View

Finite Element simulation on Abaqus ?

Hello, I am trying to simulate a triaxial test on a rock that has different materials properties. My target is to prove that by increasing the number of cells in the model, I will get more...

17 May 2019 9,772 3 View

Is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

14 January 2019 1,362 3 View

Is it better to measure secreted PCSK9 from medium cultured HepG2 cells or doing western blotting?

I want to treat the HepG2 cells with a drug that down regulates the expression of PCSK9. for assay the result, which method is better?

12 January 2018 7,303 2 View

Does anyone have protocol for lentiviral transduction of HepG2 cell lines?

Hi I need to generate TR-beta1 overexpressed HepG2 cell lines. I could not find any protocol for HepG2 transduction by lentiviral system.

30 December 2017 560 2 View

How can I choose the best plasmid vectors for lentivirus production that have my interest gene?

Hi I want to generate TR-beta overexpressed HepG2 stable cell line. For generating a lentiviral vector that have my interest gene insertion (TR-beta), that have puromycin or blasticidin...

26 December 2017 6,465 3 View

How can I get access to matinspector software for promoter analysis?

every site that I have seen, just is tutorial. Is there matinspector online tool? Is it free?

27 August 2017 4,534 4 View

What are the ethical guidelines for cell line international transportation?

I mean are there any specific codes or regulations in order to govern cell line and genetic materials international transportation?

06 March 2015 6,271 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View