Khadijeh Mahboobnia

9 Questions 13 Answers 0 Followers

Questions related from Khadijeh Mahboobnia

What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

I want to treat the HepG2 cells with either T3 or GC1 (TR-beta1 agonist) for 24 hours. I'm not sure about the internal control for qRT-PCR, in order to accurate normalisation of gene...

21 July 2019 5,891 3 View

How can I reference a translated book that I have contributed as part of a team work in order to add to my CV publication list?

I'd like to add a translated text book which its translation has been done by me and other contributers , to my publication list of my CV. I'm not sure about how could I reference that? Shall I...

02 June 2019 2,005 6 View

Is it better to measure secreted PCSK9 from medium cultured HepG2 cells or doing western blotting?

I want to treat the HepG2 cells with a drug that down regulates the expression of PCSK9. for assay the result, which method is better?

13 January 2018 7,316 2 View

Does anyone have protocol for lentiviral transduction of HepG2 cell lines?

Hi I need to generate TR-beta1 overexpressed HepG2 cell lines. I could not find any protocol for HepG2 transduction by lentiviral system.

31 December 2017 574 2 View

How can I choose the best plasmid vectors for lentivirus production that have my interest gene?

Hi I want to generate TR-beta overexpressed HepG2 stable cell line. For generating a lentiviral vector that have my interest gene insertion (TR-beta), that have puromycin or blasticidin...

27 December 2017 6,478 3 View

How can I make TRα-overexpressing hepatoma cell line (HepG2-TRα) or TR-beta overexpressing hepatoma cell line (HepG2-TR-b)?

For studying the role of these receptors in my gene of interest regulation in liver I need to have these cell lines

20 September 2017 4,069 1 View

How can I get access to matinspector software for promoter analysis?

every site that I have seen, just is tutorial. Is there matinspector online tool? Is it free?

28 August 2017 4,547 4 View

For promoter analysis for finding the transcription factor binding sites, which on line tools are the best?

Is there any tutorial file for JASPAR?

01 August 2017 8,596 8 View

Why control antibody like anti-GFP antibody should be used for IP technique?

why control antibody like anti-GFP antibody should be used for IP technique?

22 December 2016 7,951 4 View