I am working with Uvsx Recombinase having conc as 5 microgram / microliter. It has 10XUvsx Recombinase reaction buffer. Total amount of recombinase is 100microgram.
Hi there,
To make a good dilution first you need to know the dilution factor you want to apply... This dilution factor is the ratio between initial and final concentrations (expressed in the same unit). In your case it's 5/0.12= 41.67. Which means that to prepare your dilution you will have to put 1 part of the starting solution in 40.67 parts of diluent (for instance 10µL in 406.7µL of diluent). So depending on your actual need, you have to set the final volume... One big issue with your recombinase dilution is : what diluent should you use? The only buffer you have in the 10x reaction buffer which has to be diluted 10x when preparing the mix for the recombinase assay. The question is should the dilution be 1x toward the reaction buffer? If so the dilution will then contain 1 part of stock recombinase, 4.167 part of 10x buffer (1/10th of the final volume) and (41.67-1-4.167)=36.5 part of water.
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