What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What is the role of quencher in ROMP?, is its quantity decided according to monomer concentrtion undergoing polymerization or from the catalyst?

In ROMP M:C is 100:1, so whats the ratio of Quencher be added in? will quencher precipitate the polymer?

01 December 2020 1,283 4 View

How to Improve the output of Supercapacitors To be used in Elctric Vehicles?

Supercapacitors are the future they say but is there any way to use them only without any battery along. As I have read that MIT is doing research for the development of this but there has been no...

10 November 2020 3,816 2 View

Protein extraction from yeast?

I am having difficulty finding the protein from yeast? Just want to extract protein and detect it by biuret reagent methods. I stored my yeast samples at -20C. So my question is how can I rupture...

18 February 2020 2,717 9 View

How to check if the water samples has ethanol in it?

I am growing yeast aerobic condition. I need to test if the yeast is producing alcohol during the growth. After separating the yeast through filter paper, the medium is just water. Is there any...

23 October 2019 1,870 2 View

Microorganism growth and Substrate removal?

I have grown Yeast in synthetic medium. In two days all the substrate were consumed, However, the growth was observed until day 5 and becomes stationary after day 5. What could be the possible...

09 June 2019 8,823 3 View

What are the key differences among commonly used Travel Demand Forecasting software in Transportation Engineering?

01 October 2017 6,665 4 View

Can it be different solution with different relaxation factors with simple algorithm in fluent?

I am running a fluid flow problem in channel, but facing problem with different solutions with different relaxation factors. 1). Initially i set default relaxation factors, then residual curves...

29 January 2016 6,453 3 View

No title

Software for multi-lingual Qualitative data analysis

14 September 2014 7,455 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View