Akshyaya Rath

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Questions related from Akshyaya Rath

Does higher concentration of Tween 20 in washing buffer in indirect ELISA may remove the antigen coating from the plate?

I am using 0.25% Tween 20 in PBS as wash buffer in an indirect ELISA for antibody titre estimation. I am getting variation in internal quality control. Can the variation is due to concentration of...

19 August 2021 195 4 View

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA?

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA? The OD values obtained at different dilution how to determine the cut off OD against which the antibody titre...

01 February 2020 1,040 4 View