Akshyaya Rath

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Questions related from Akshyaya Rath

How can I make 0.05 molar phosphate citrate buffer of PH 5?

Please give complete recepie and preparation protocol

23 December 2023 3,071 0 View

Does higher concentration of Tween 20 in washing buffer in indirect ELISA may remove the antigen coating from the plate?

I am using 0.25% Tween 20 in PBS as wash buffer in an indirect ELISA for antibody titre estimation. I am getting variation in internal quality control. Can the variation is due to concentration of...

19 August 2021 254 4 View

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA?

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA? The OD values obtained at different dilution how to determine the cut off OD against which the antibody titre...

01 February 2020 1,198 4 View

During hepatocyte isolation from high fat fed mice does the high liver TG levels effect the process?

During hepatocyte isolation from high fat fed mice. The media become so hazy that the cell separation could not be marked even after centrifugation during the washing. Gradually the cells clumped....

29 April 2017 3,652 0 View