Hi,
I have been injecting AML primary cells in NGS-SGM3 mice and they developed leukemia. After recovering the human AML leukemic cells from the bone marrow it is difficult for me to keep them in culture and especially to expand them. I am using SFEM media with CC100 cytokine cocktails + Tpo. This media worked well for primary AML but is not giving me good results with the same cells isolated from the mice/xenograft models.
How can I optimize the condition to maintain AML cells recovered from the mice?
Thanks
Patrizia
Giuseppe Germano
Hi Patrizia,
we use a medium that is composed as follows: RPMI with 10% FBS, cytokines as IL3, IL6 (20 ng/ml) and SCF, TPO, FLT3L (50 ng/ml). In general, the cells survive but not duplicate efficiently as you would expect.
Giuseppe
0 votes
0 thanks
- Badges
- Science method
Similar questions and discussions