How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View

How to do a Repeated measures analysis with not normal distributed data?

I have results from a listening test in 12 sound environments which are build up out of 3 room properties and 4 occupancy rates. Since the sound samples were often intelligible, subject scores...

04 November 2020 5,423 11 View

Can I use my DNA after the tube was "sterilized" by UV light?

I left tubes with isolated DNA drying (before resuspension) in a biosafety hood for too long and a colleague came and turned on the UV light for some 20-30min. The light did not shine on the DNA...

29 September 2020 536 3 View

How could I avoid mutations upon transforming my gRNAs into the lentiCRISPRv2 vector?

After I have cloned my gRNAs into the lentiCRISPRv2 vector and sent the construct for sequencing, I have noticed that a single mismatch between my clone and original sequence has been introduced....

11 September 2020 4,066 1 View

DNA extraction of swabs stored in ethanol?

Hi all, I am using buccal swabs of seals as a sample source for microsats and mitochondrial sequencing. There is a few samples that were shipped stored in ethanol. , i.e. swab of the buccal area...

08 September 2020 1,013 1 View

What's the best way to image cancer tumor growth other than FDG?

Was quantifying clinical chemotherapeutic efficacy with FDG (radioactive glucose). This general read of metabolism a good match for highly metabolically active cancer cells. However, some...

18 February 2020 6,845 1 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How do I interprete the results of a moderation analysis with a categorical and a continuous variable?

Hi all, I have a question concerning a moderation analysis within an experimental setting. The independent variable is categorical and has two options. The moderator is a continuous variable. I...

06 January 2020 9,511 1 View

How to run a binary logistic regression with two experimental treatments?

Hi all, is it possible to run a binary logistic regression within an experiment? That means that the two experimental treatments (treatment 1 vs. control) predict a categorical variable...

03 December 2019 7,319 9 View

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Does anyone have a protocol or experience with extracting genomic DNA from very small sample volumes of 10-30 microliters? The sample is a microbial enrichment from a sewage treatment plant.

30 September 2019 6,973 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View