i want to do the ion exchange chromotography. pI value of my protein is 5.1.
i am working on a transporter protein and now i need to check its ATPase activity. i have searched and read so many protocol but i got really confused with the addition and subtraction of some...
09 October 2018 6,040 2 View
i am going to perform a glycosylation assay on my protein. do i need to run my protein on native gel? how periodic schiff reagent should be use for this procedure ?
31 December 2017 335 1 View
i am working on esterase and need to know its in silico interactions with different substrates
10 November 2017 9,198 2 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...
24 February 2021 5,974 4 View
Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...
21 February 2021 5,614 3 View
Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...
21 February 2021 8,132 1 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!
09 February 2021 6,202 1 View