How can I isolate topoisomerase 1 enzyme from plasmodium cell? Is there any define protocol, please suggest?

More Pooja Rani Mina's questions See All

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Does the China's adaption to GM soybean Technology contradicts to its practice of virtual water trade policy?

Recently, the Ministry of Agricultural and Rural Affairs enacted the approval of twelve genetically modified (GM) soybean varieties, marking a strategic pivot in China's quest for food security....

05 June 2024 1,143 0 View

How can I adjust the cell number (cfu/ml) of Pseudomonas aeruginosa ATCC 27853 to OD600 nm absorbance and the McFarland standard?

Hi everyone Good time. I am working on Pseudomonas aeruginosa ATCC 27853 in our lab and I want to know whether there is any strong literature or previous data about the correlation between the...

13 May 2024 6,888 0 View

How can I correct the orientation of GOI from one plasmid to another?

A plasmid of mine has GOI in opposite orientation. I have another plasmid which has exact same RE site but in such a way that it orients my GOI in right direction. However even after multiple...

05 May 2024 5,662 2 View

What do you advise me?

I want to read a book about human resources management. What do you advise me?

27 April 2024 2,645 3 View

How to find covariance matrix in case of parameter estimation by using Recursive least square with forgetting factor?

Thank you for reading my question. Currently, I am doing parameter estimation of Lithium-ion battery by using RLSF Algorithm. I am getting all the parameters as negative value. Which is not...

17 April 2024 8,941 0 View

Can anyone advice me on LncRNA primer designing for cloning to study its overexpressed pattern in the cell?

I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as...

14 April 2024 3,966 3 View

How important is AI for marketing?

How can AI improve marketing for businesses?

19 March 2024 2,867 5 View

Solvent for mucilage other than water?

Please recommend any organic solvent for dissolving mucilage.

30 January 2024 5,692 5 View

I have a mixed microbial suspension. I need to analyze all the enzymes, if any, present in the sample. Can anyone suggest which technique will work?

Which process or technique will work? Will LC-MS give me a full enzyme profile?

26 January 2024 3,374 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Pooja Rani Mina

sir can you upload full article because i cant access this page pdf

Adam B Shapiro

Is it necessary to isolate the protein from the native source, i.e. Plasmodium cells, or would it be sufficient to overexpress and purify the protein heterologously using the cloned gene and purify the protein?

Naser Jawad Kadhum

https://www.beiresources.org/portals/2/MR4/Methods_In_Malaria_Research-6th_edition.pdf