I am trying to isolate DNA from Thrips. DNA isolation kits are not for single DNA thrips isolation.
I use Qiagen - DNeasy Blood & Tissue Kit for DNA isolation of single thrips specimens. I recommend you to crush the insect with micropestle in the ATL buffer and to finish the extraction using the producers protocol. Good luck!
Thanks for your valuable suggestion, I will follow the same....
if you have small amout of starting material, you can try this one-works really good:
http://www.qiagen.com/Products/Catalog/Sample-Technologies/DNA-Sample-Technologies/Genomic-DNA/QIAamp-DNA-Micro-Kit
Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India
04 February 2021 8,632 8 View
I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.
19 January 2021 1,136 3 View
Sympatric birds may avoid competition via multiple strategies - by different activity period, different diet, different microhabitat etc. Has any one looked into behavior of sympatric birds as...
11 January 2021 9,600 1 View
Respected all, Can anyone help me regarding where I can get complete and proper tutorials on IBM CPLEX Optimization software? I found some tutorials on Youtube...but they are just basic. I need...
05 January 2021 6,751 6 View
Gao (1996) gave the expression to calculate NDWI using Near Infrared (NIR) and Short wave Infrared (SIR), while some research suggests the use of the Green (G) band and Near Infrared (NIR) bands....
28 December 2020 9,938 2 View
the area received high rainfall, only last 3 months of hydrological year faces acute water shortage even for drinking the solution for this may be rainwater harvesting now how value...
25 December 2020 2,408 4 View
Interested in Reaserch to join steel material with carbon fiber for better stiffness and mechanical properties.
19 November 2020 9,790 2 View
(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...
06 October 2020 9,496 5 View
Is 8th week the earliest week where we can determine the fetal sex ?
05 October 2020 9,576 9 View
While learning action recognition we need to exploit both global and local temporal features. But cant understand the difference between the two??
03 August 2020 9,899 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View