Dear Mr. Amarathunga
Regards
Several methods are available in order to isolate microalgae from natural sources. All depends on your expertise and materials available. A good review about these techniques is presented in follow document.
https://www.researchgate.net/file.PostFileLoader.html?id=5586b0815dbbbdb49c8b45af&assetKey=AS%3A273799340068866%401442290257621
please go through this reference, it will help for your stuy.
Stein J. R. (1973). Handbook of phycological methods: Culture methods and Growth measurements. Cambridge University Press, London and New York. 448pp.
Dear Deeptha:
You can get a sample of water and do enrichment first (if the algal population is low).
Enrichment: get water sample and place it in BG 11 medium or Bold's Basal Medium
High concentration of algal cell in water sample - you can do serial dilution and spread plating in BG 11 medium...usually typical colonies of Chlorella appear green in the agar media.
I prefer to read the following book:
Algal Culturing Techniques, 2005. Edited by Robert A. Andersen.
If you not get it please send me an email.
Dear Kumaresan,
I had sent to you via email the book.
All the best
Fikrat
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