hello Safaa Nassar

99% of the bacteria in the soil cannot live on media in petri dish

because they are aerobic and this technic is only used for anaerobic bacterias

so when u make isolate from soils on petri dishes you will only get facultative anaerobic and anaerobic bacteria (which are the disease bacteria)(a simple dextrose agar would do the job) this will lead you to false results if you are studying the biodiversity of the bacteria in the rhizosphere

instead i suggest a microscopic analysis or pcr to study the diversity of bacteria in the rhizosphere

i would love to learn more about your project

also i'm ready to answer further questions you have

Hi,

You can use GYM media ( glucose, yeast extract, malt extract) for actinobacteria, and TSA ( Tryptic soy agar).

Hi Safaa,

As far as I know there is no medium that in which all bacterial spectrum can grow in it. this is why you need to know what kind of Rhizobacteria you are targeting. It seems that you want to detect the bacterial spectrum that are resistant to the used antibiotics. I recommed you some media that you can use so that you have a comprehensive idea of you rhizobacteria population.

as rich media: you can use LB (luria bertani), NB(nutrient Broth) and TSA.

as selectif media: you can use Jensen`s medium, YMA medium and PVK medium( these media are used to detect Plant Growth Promoting Rhizobacteria)

I hope this answer brought you some help.

feel free to contact for more details.