How can I insert short sequences by isothermal assembly?

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Vegard Eldholm

Hi!

Maybe it could work?

But if you need to insert such a small piece, why not add it to the 5' of a primer and introduce it to a larger part by pcr?

Cheers,

Vegard

Syed A Ali

Hello Lorena,

Unless it is absolutely neccessary to go for isothermal assembly, why not restrict your plasmid with two REs and clone your 50 bp insert employing anneald oligo cloning?