Hello Carlos Arias-Mainer

You may start with a concentration as low as 20uM of H2O2 and go up to 400uM.

Best

Hello Malcolm Nobre,

Thanks for your reply.

Do you know how long it takes for cells get stressed? I have read about 10-30 minutes of exposure time with H2O2 at concentrations of 20-100 uM.

Do you know if after 10-30 minutes of exposure time, cells are already stressed to perform the assay?

Thanks.

Hi Carlos Arias-Mainer , I am currently looking to induce oxidative stress on 3T3-L1 adipocytes using H2O2 too. I was wondering what concentration and incubation period worked best for you?

Thanks.