What may be the contaminant observed in animal cell culture ?

The following image uploaded is from our Sub-ventricular Zone (SVZ) derived neurosphere culture (mice), Primary culture - Passage 2 - Day 2. Thread like structures were observed at 20X and 40X....

27 June 2018 3,269 10 View

Does Glutamine free medium contain Glutamate?

I want to use glutamate free medium for my experiment but I could not find it. But the glutamine free medium is commercially available and I want to ask whether glutamine free medium contains...

12 June 2018 8,237 3 View

How to preprocess multispectral image with low pixel values in shaded areas?

I have WV 3 image with very low pixel values in the shaded areas. Image covers a very large area and it is very clear. I am trying to do radiometric and atmospheric correction (ENVI FLAASH and...

28 December 2017 5,631 3 View

Can somebody please inform me about the availability and cost of ELISA kits for measuring rat testosterone, estrogen, progesterone, FSH and LH?

10 July 2014 3,304 3 View

What are the controls for immunoprecipitation that will be excised and sent for MS for protein detection?

My protein is tagged with HA. I plan to use magnetic beads. Can anybody suggest me.

27 June 2013 401 2 View

Two separate bands for PCR products of a same gene on a gel!

Hi Why two different bands appear for PCR products of a same gene, when run them in a same gel, same time (master mix were prepared using same reagents and Cdna). I had quadruplicates in PCR run...

01 January 1970 4,392 3 View

What are the high yielding methods of extraction of phycocyanin from dry mass of Anthirospira plantesis?

extraction techniques

31 January 2020 7,268 1 View

A lake I am monitoring experienced a blue green algae bloom followed immediately by a green algae bloom. Any insight on why this would occur?

I have YSI sensors in a lake I am monitoring, which feed chlorophyll and phycocyanin readings to me on a regular basis. Awhile back I noticed a spike in the phycocyanin reading. As the peak was...

06 July 2019 148 3 View

What's the fastest way to extract phycocyanin from Spirulina?

I am going to extract phycocyanin for a nanotechnology project. What is the most cost-effective and fastest way to do that?

03 April 2019 1,407 5 View

Can any one share the protocol of Phycocyanin isolation from Spirulina following Freeze and thaw method?

I need the exact amount of fresh biomass to be taken,proportion of Phosphate buffer to be added, The Cycles to be followed and time taken for 1 cycle.Kindly help me in this regard.

11 December 2018 8,242 0 View

I am working with extraction of phycocyanin. Can anyone suggest me what all work can i do with crude phycocyanin except molecular biology?

future perspective of phycocyanin

11 December 2017 797 3 View

How to thaw samples?

11 December 2016 7,408 4 View

How to calculate phycobiliproteins, total R-PE and total R-PC (mg/mL) using formulae?

I'm trying to calculate phycobiliproteins, total R-PE (R-phycoerythrin) and total R-PC (mg/mL) using formulae but i noticed as the extraction getting more purified; the higher the absorbance will...

07 August 2016 6,939 6 View

Can anyone suggest an impact factor journal for chemopreventive activities of phycobiliproteins with very fast reviewing time?

I need to publish an article about phycobiliproteins and their chemopreventive potential. Please suggest an impact factor journal with less review time and high chances of my paper to get publish.

06 July 2016 1,784 2 View

What are the effective and suitable methods to separate PEG and purified phycocyanin in the ATPS?

01 February 2016 7,674 0 View

Does anyone have experience differentiating marine bacteria using flow cytometry?

18 October 2015 5,575 4 View