I characterized my materials by PL spectroscopy. I observed some enhancement in my emission intensity. From this data can I calculate Quantum yield? If anybody know the calculation please give the notes.
If you know the quantum yield, Qi, of your material before the "enhancement", then you can calculate the quantum yield, Qf, of your material after the "enhancement":
Qf = Qi x If / Io
where If is the emission intensity after enhancement at a particular emission wavelength (for example, the peak maximum) and Io is the emission intensity before the enhancement at the same wavelength.
If you do not know the quantum yield of your material before the enhancement, you will need an actinometer to calibrate your spectrometer. The actinometer is a chemical or device that can be used to determine the photon flux in your spectrometer. In your case, you can use a fluorescent solution whose quantum yield, Qa, is known and whose excitation wavelength is similar to that of your test materials. The quantum yield of your sample, Qs, can then be calculated using:
Qs = Qa x (Is / Ia)
where Is is the emission intensity of your sample at the wavelength you wish to determine the quantum yield and Ir is the emission intensity of the actinometer at wavelength at which it was standardized.