Science Red seaweeds have a good quantity of water-soluble phycobiliprotein, which may precipitate during extraction with some of the solvents. Thus, I am searching for a better solvent to estimate the antioxidant activities of red seaweeds.
Ethanol/water v:v (50:50)
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I need a detailed protocol of any suitable method for ex., DPPH to determine antioxidant activity.
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My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...
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Are there depletion experiments for these three antioxidants in Atlantic Salmon?
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and Why do double bonds reduce it ?
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I have solubilized DPPH in methanol and used ascorbic acid as a positive control, and am testing the activity of pectins.
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As a context, we have been optimizing the DPPH protocol for our seaweed ethanolic extract. We also use varying concentration points from the various methods in published journal articles, yet none...
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