I needed to do vagotomy on B6 mice so I followed this protocol:
http://www.ncbi.nlm.nih.gov/pubmed/23943456
However, the mice died in one day, sometimes even after 15 minutes. I need to establish this model to test whether the effect is through the parasympathetic nerve to the heart. Could anyone give me some suggestions on how I could improve the mouse survival rate? Dissect unilateral? Left or right?