Hi all,
There are obstacles that I have faced during electrophysiology recording for the neurons derived from stem cells. Neurons were fell out once I started patching cells. I cannot record any actional potential and/ or sodium or potassium current. Although I have good neurons in my culture. I would appreciate it if you can give me advice to overcome this issue. Thank you.
Andre Dagostin
Hi Randah. Cultured neurons can be a little finicky to work with. From what I understood, you can't get anything from them. So let me ask: how is the seal (leak current)? And the resting membrane potential in current clamp? Did this preparation work smoothly before? What's the Na concentration in your culture media?
Andre
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