How to precede after obtaining gene-exp.diff from cuffdiff for RNA-seq analysis ?

Hello , I am new to RNA-seq and can anyone guide me on how to do analysis on my data? I know I need to write a script to select my data based p-values and Q values for differential expression,...

22 March 2020 8,995 5 View

What is the most proper, cheap and reliable device(portable) which measure HRV for scientific research? Are Polar watchs or other arm bands reliable?

Hello. I want to measure HRV before activity, during activity and after activity. Each measurement will take 5 minutes. I have limited budget and i m searching for a portable device. And it must...

07 March 2020 4,807 3 View

How to do Bland Altman Plot in measurement pairs in SPSS or Excel?

Hi all, Although I watched all the videos about Bland Altman plot in SPSS, I could not find any information regarding how to use this statistics in multiple replicates for each method. Hope...

13 September 2018 9,359 14 View

Detecting homologous regions (hrs) in Lymantria dispar nucleo polyhedrovirus ( LdMNPV )

I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...

05 July 2017 3,752 2 View

For a mathematician, is it important that: Does a subject in math have any application in other areas?

06 April 2017 7,979 5 View

How can we evaluate mitochondrial oxidative stress?

I want to analyse oxidative status in mitochondria of fish liver tissue.

26 February 2015 3,660 6 View

Is there any relationship between transmembrane domains and 3' UTR regions?

I am using Roche 3'/5' 2nd generation RACE kit to determine 3' and 5' utr regions. I have completed all steps and sequenced my 3' plasmids (pGEM-T Easy vector). The main point: my gene has a...

09 June 2014 2,499 1 View

Fabrication of Photonic Crystals?

Does anyone knows how to fabricate Photonic crystals by using TiO2?

04 February 2014 3,399 5 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View