How to precede after obtaining gene-exp.diff from cuffdiff for RNA-seq analysis ?

Hello , I am new to RNA-seq and can anyone guide me on how to do analysis on my data? I know I need to write a script to select my data based p-values and Q values for differential expression,...

22 March 2020 8,995 5 View

What is the most proper, cheap and reliable device(portable) which measure HRV for scientific research? Are Polar watchs or other arm bands reliable?

Hello. I want to measure HRV before activity, during activity and after activity. Each measurement will take 5 minutes. I have limited budget and i m searching for a portable device. And it must...

07 March 2020 4,807 3 View

How to do Bland Altman Plot in measurement pairs in SPSS or Excel?

Hi all, Although I watched all the videos about Bland Altman plot in SPSS, I could not find any information regarding how to use this statistics in multiple replicates for each method. Hope...

13 September 2018 9,359 14 View

Detecting homologous regions (hrs) in Lymantria dispar nucleo polyhedrovirus ( LdMNPV )

I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...

05 July 2017 3,752 2 View

For a mathematician, is it important that: Does a subject in math have any application in other areas?

06 April 2017 7,979 5 View

How can we evaluate mitochondrial oxidative stress?

I want to analyse oxidative status in mitochondria of fish liver tissue.

26 February 2015 3,660 6 View

Is there any relationship between transmembrane domains and 3' UTR regions?

I am using Roche 3'/5' 2nd generation RACE kit to determine 3' and 5' utr regions. I have completed all steps and sequenced my 3' plasmids (pGEM-T Easy vector). The main point: my gene has a...

09 June 2014 2,499 1 View

Fabrication of Photonic Crystals?

Does anyone knows how to fabricate Photonic crystals by using TiO2?

04 February 2014 3,399 5 View

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23 February 2021 4,014 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How do I dissolve a TPU (Texin RxT85A) in THF?

I want to make a 6% solution of Texin RxT85A in THF. I weighed the necessary amount of Texin resin and mixed at room temperature with THF with the help of a wooden stick, this did not work at all....

18 January 2021 9,080 3 View

Is there an alternative, more ecological solvent for shape memory polyurethane other than N, N-dimethylformamide (DMF) ?

As DMF creates problems for environmental aspects, we are looking for an alternative solvent for potential commercial end use applications of SMPU.

25 November 2020 8,728 3 View

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When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

What does over foaming correspond to in E.coli fed-batch fermentation?! How does foaming relate to cells metabolic status?

I'm currently experiencing overfoaming in my E.coli culture. My cells are used to produce a recombinant protein. The basic cell strain is BL-21 DE3. After changing my carbon source brand i am...

06 October 2020 3,946 3 View

What is the influence of the mold release agent on sound absorption properties of polyurethane foam ?

I'm an intern in a big company which makes flexible polyurehane foam for the automotive sector and I'm working on sound absorption properties of PU foam. I'm not chemist so I'm trying to improve...

23 September 2020 3,765 4 View

Which damage criteria should be used for nonlinear viscoelastic behavior?

Hello, I am building a material model for a nonlinear viscoelastic polymer in ABAQUS. For the beginning I just want to simulate an uniaxial tensile test. But now I want to know which damage...

13 September 2020 6,606 4 View