I am currently trying to work with the epithelial lung cancer cell line CALU3 cells for a transfection study and finding that my cultures are taking an extraordinarily long time to get to even 50% confluency (approximately a week in some cases).
At present I am using the following reagents to grow primary cultures:
- DMEM containing 14.5 g/L glucose, L-glutamine + sodium pyruvate (Corning, 10-013-CV)
- 1% Penicillin Streptomycin (Gibco, 15140122 )
- 20% Heat inactivated fetal bovine serum (Gibco, 16140071)
Though this is my first time working with CALU3, I am aware that it is a rather divergent cell line and can vary from lab to lab in terms of physical characteristics. Perhaps the line I inherited just has a slower growth cycle? Do my media and additives look insufficient?
Dear Madeline Clark
If you are still struggling with the cells then perhaps you can increase the seeding density.
I have observed that they grow better/faster when seeded at a higher density.
Although I should mention that my culture conditions are not the same as yours. I culture them without pen/str and only 10% FBS. So there might be differences due to that as well.
Good luck !
Dear Shambhabi Chatterjee Do you face bacterial contamination issue in Calu-3 cells? Not immediately, but after certain passage?
Hi Utkarsh Bhardwaj
No, i have not experienced bacterial contamination during Calu-3 cell culturing (atleast in time frame of 6 months).
Can you please share the media composition which you use? Shambhabi Chatterjee
I culture Calu3 cells in DMEM (https://www.thermofisher.com/de/de/home/technical-resources/media-formulation.170.html ) media containing 10% FBS.
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